Characteristics and metabolic pathways of fluorene (FLU) degradation by strain Rhodococcus sp. USTB-C isolated from crude oil

نویسندگان

  • Chan Yu
  • Jun Yao
  • Jingnan Jin
چکیده

A fluorene-degrading strain named USTB-C was isolated from crude oil of production well in Dagang oil field, southeast of Tianjin, northeast China. The 16S rDNA sequence of USTB-C showed 100% similarity with that of Rhodococcus erythropolis PR4, it was belonged to Rhodococcus. The strain USTB-C could remove 55.8% of fluorene with an initial concentration of 100 mg L in 23 days without other substrates. It implied that the strain USTB-C showed the potential to degrading efficient of fluorene. Based on the intermediate metabolites analyzed by gas chromatography-mass spectrometry (GC-MS), we deduced the possible metabolic pathway of strain USTB-C for fluorene biodegradation. Fluorene was initially oxidized at C-9 positions finally resulting 9-fluorenone. Through hydrolysis reaction, 9-fluorene subject to form phthalic acid. Furthermore, phthalic acid was further degraded to1 -hydroxybenzene. USTB-C evidently possesses efficient, high effective degrader and potential for further application on the enhanced bioremediation technologies for treating fluorene-contaminated soil.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Novel Bacterial Strains Pseudomonas sp. and Bacillus sp. Isolated from Petroleum Oil Contaminated Soils for Degradation of Flourene and Phenanthrene

Flourene and phenanthrene are organic compounds with high hydrophobicity and toxicity. Being recalcitrant in nature they are accumulating in the environment at an alarming concentration, posing serious threat to living beings. Thus in the present study, microorganisms were screened for their ability to degrade these contaminants at high concentrations in least period of time. Two out of fifteen...

متن کامل

Novel Bacterial Strains Pseudomonas sp. and Bacillus sp. Isolated from Petroleum Oil Contaminated Soils for Degradation of Flourene and Phenanthrene

Flourene and phenanthrene are organic compounds with high hydrophobicity and toxicity. Being recalcitrant in nature they are accumulating in the environment at an alarming concentration, posing serious threat to living beings. Thus in the present study, microorganisms were screened for their ability to degrade these contaminants at high concentrations in least period of time. Two out of fifteen...

متن کامل

Statistical Optimization of Crude Oil Biodegradation by Marinobacter sp. Isolated from Qeshm Island, Iran

Background: Hydrocarbons degradation is principally achieved by microorganisms in natural environments. The extent of hydrocarbons biodegradation is mainly conditioned by environmental factors and its success depends on the optimal condition for the crude oil degrading isolates. Objectives: The aims of the current study was to isolate and identify crude oil degrading bacterium from surface sed...

متن کامل

ارزیابی تجزیۀ زیستی آنتراسن به وسیلۀ Gliomastix sp. جداشده از خاک های آلوده پالایشگاه شازند، ایران

In this study, fungal strains with crude oil biodegradation activity were screened from Shazand oil refinery (Arak). Twelve fungal strains were isolated in PDA medium. TPH assay in the presence of 1% of crude oil showed that the ADH-02 was the most capable strain of oil degradation with an efficiency of 75%. FTIR analysis was revealed that 91% of aliphatic hydrocarbons were degraded by ADH-02. ...

متن کامل

Bioremediation potential of a phenol degrading bacterium, Rhodococcus erythropolis SKO-1

Biodegradation of phenol is a major focus of toxic organic compound degradation by microorganisms isolated from polluted areas. An increasing number of bacteria and fungi possessing unique biodegradation capabilities have been isolated in recent years. In this study a new isolate, Rhodococcus erythropolis SKO-1, from polluted soils in the Tehran oil refinery region, is reported. Identificati...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2014